Which agar is unsuitable for culturing Vibrio cholerae?

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Cefsulodin-irgasan-novobiocin (CIN) agar is specifically designed for isolating Yersinia enterocolitica and is not ideal for culturing Vibrio cholerae. This medium has selective agents that inhibit the growth of Vibrio species while promoting the growth of Yersinia. Therefore, it is unsuitable for the isolation of Vibrio cholerae, which requires more conducive conditions and different selective media to thrive.

In contrast, MacConkey agar, TCBS Agar, and Buffered charcoal yeast extract are suitable for culturing Vibrio cholerae. MacConkey agar can support the growth of Gram-negative bacteria, including V. cholerae, although it is primarily tailored for Enterobacteriaceae. TCBS agar is specifically formulated for isolating vibrios, providing a very favorable environment for V. cholerae through the appropriate balance of bile salts and sucrose, which promotes its growth. Buffered charcoal yeast extract is especially useful for growing fastidious organisms, though it is less specific than TCBS for Vibrio species. Therefore, B stands out as the answer that identifies an unsuitable medium for Vibrio cholerae.

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